|
Marine bacterium, as a microbial source producing polysaccharides, was newly isolated from the eastern and western sea of Korea and was identified as Zoogloea sp. (KCCM 10036). It produced two different types of polysaccharides, especially : WSP (water-soluble polysaccharide) and CBP (cell bound polysaccharide). The former was isolated from the supernatant of centrifuged broth by acetone precipitation, and the latter was isolated from the pellet by acetone and CPC (cetylpyridinium chloride) precipitation. The productivities of polysaccharides were increased with the addition of promoting agents such as biotin, ampicillin and surfactant. After batch fermenting, the productivities of WSP and CBP were reached to maximum values of 9.0g/P , 2.5g/~ in the culture medium containing 1 % of glucose as a carbon source.
|